منابع مشابه
Degenerate DNA recognition by I-PpoI endonuclease.
The I-PpoI endonuclease is encoded by a group I intron found in the slime mold Physarum polycephalum. To initiate homing of its encoding intron, I-PpoI catalyzes a specific double-stranded break within a 15-bp recognition site. The high substrate specificities of I-PpoI and other homing endonucleases make these enzymes valuable tools for genomic mapping and sequencing. Here, we report on the ab...
متن کاملStructural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease
Restriction endonuclease Bse634I recognizes and cleaves the degenerate DNA sequence 5'-R/CCGGY-3' (R stands for A or G; Y for T or C, '/' indicates a cleavage position). Here, we report the crystal structures of the Bse634I R226A mutant complexed with cognate oligoduplexes containing ACCGGT and GCCGGC sites, respectively. In the crystal, all potential H-bond donor and acceptor atoms on the base...
متن کاملSeismic Data Forecasting: A Sequence Prediction or a Sequence Recognition Task
In this paper, we have tried to predict earthquake events in a cluster of seismic data on pacific ring of fire, using multivariate adaptive regression splines (MARS). The model is employed as either a predictor for a sequence prediction task, or a binary classifier for a sequence recognition problem, which could alternatively help to predict an event. Here, we explain that sequence prediction/r...
متن کاملDegenerate peptide recognition by Candida albicans adhesins Als5p and Als1p.
Candida albicans and Saccharomyces cerevisiae expressing the adhesins Als5p or Als1p adhere to immobilized peptides and proteins that possess appropriate sequences of amino acids in addition to a sterically accessible peptide backbone. In an attempt to further define the nature of these targets, we surveyed the ability of yeast cells to adhere to 90- micro m-diameter polyethylene glycol beads c...
متن کاملRepression of antibiotic downregulator WblA by AdpA in Streptomyces coelicolor.
The upstream region of antibiotic downregulatory wblA in Streptomyces coelicolor was found to contain AdpA binding motifs. A key morphological regulator, AdpA was shown to specifically bind these motifs by electrophoretic mobility shift assay. An adpA disruption mutant exhibited increased wblA transcription, suggesting that AdpA negatively regulates wblA transcription in S. coelicolor.
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations and Advances
سال: 2014
ISSN: 2053-2733
DOI: 10.1107/s205327331409785x